Comparison of the X-ray structure of native rubredoxin from Pyrococcus furiosus with the NMR structure of the zinc-substituted protein.

نویسندگان

  • P R Blake
  • M W Day
  • B T Hsu
  • L Joshua-Tor
  • J B Park
  • D R Hare
  • M W Adams
  • D C Rees
  • M F Summers
چکیده

The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability.

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protein zinc - substitutedPyrococcus furiosus with the NMR structure of the Comparison of the X - ray structure of native rubredoxin from data

The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 "C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 "C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hype...

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عنوان ژورنال:
  • Protein science : a publication of the Protein Society

دوره 1 11  شماره 

صفحات  -

تاریخ انتشار 1992